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CABRI Guidelines

LABORATORY PROCEDURES FOR PLANT CELL LINES

Part 1: Introduction and general documents

Procedures

Appendixes

Part 2: Request and handling new deposits

Flow diagram

Procedures

Appendixes

Part 3: Maintaining deposits

Procedures

Appendixes

Part 4: Delivery of material to the customer

Procedures

Appendixes


Part 1: Introduction and general documents - Procedures -

Reference Nb.

Procedure Title

PC/1998/1/1

General Guidelines for Management and Organisation

PC/1998/1/2

General Principals and Guidelines

PC/1998/1/3

International Laws and Regulations Concerning the Collection Work

PC/1998/1/4

European Laws and Regulations Concerning the Collection Work

PC/1998/1/5

National Laws and Regulations Concerning the Collection Work

PC/1998/1/6

Security, Quality and Transfer Restriction Codes

PC/1998/1/7

Introduction to safety regulations

PC/1998/1/8

Issue, control and updating of laboratory procedures

PC/1998/1/9

Review of Laboratory Record Books and Protocol Forms

 

Part 1: Introduction and general documents - Appendixes -

Reference Nb.

Appendixes

PC/1999/1/2 Appendix 1

WFCC Guidelines for the Establishment and Operation of Collections of Cultures of Micro-organisms

PC/1998/1/7 Appendix 1

Safe Handling of Liquid Nitrogen

PC/1998/1/7 Appendix 2

First Aid for Laboratory Infections

PC/1998/1/7 Appendix 3

Good Microbiological Practice

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Part 2: Guidelines for requesting and handling new deposits - Procedures -

Reference Nb.

Procedure Title

PC/1998/2/1

Guidelines for Requesting, Processing, Cataloguing, Maintenance and Delivery of Plant Cell Lines

PC/1998/2/1.1

Flow chart: Requesting, Processing, Maintenance and Delivery of Plant Cell

PC/1998/2/2

Request and receipt of plant cell lines

PC/1998/2/2.1

Definition of Accession criteria

PC/1998/2/2.2

Description of Accession Form

PC/1998/2/2.3

Day Book

PC/1998/2/2.4

Allocation of Accession Number

PC/1998/2/3

Preliminary Quality Control

PC/1998/2/3.1

Macroscopic and Microscopic Examination

PC/1998/2/3.2

Test for Fungal and Yeast Contaminants

PC/1998/2/3.3

Test for Bacterial Contaminants

PC/1998/2/3.4

Viability test: TTC

PC/1998/2/3.5

Viability test: FDA

 

Part 2: Guidelines for requesting and handling new deposits - Appendixes -

Reference Nb.

Appendixes

PC/1998/2/2 Appendix 1

Standard letter: Request of a plant cell line

PC/1998/2/2 Appendix 2

Standard letter: Acknowledgement of Accession Form

PC/1998/2/2 Appendix 3

Standard letter: Asking for alternative methods

PC/1998/2/2 Appendix 4

Standard letter: Acknowledgement receipt of accession form

PC/1998/2/2 Appendix 5

Standard letter: Acknowledgement of receipt cell line

PC/1998/2/2.1 Appendix 1

Accession criteria

PC/1998/2/2.2 Appendix 1

Accession Form

PC/1998/2/2.3 Appendix 1

Day Book, exemple

PC/1998/2/3 Appendix 1

Standard Letter: Cell line contaminated

PC/1998/2/3 Appendix 2

Standard Letter: Low Viability 1

PC/1998/2/3 Appendix 3

Standard Letter: Low Viability 2

PC/1998/2/3 Appendix 4

Standard Letter: no Viability

PC/1998/2/3.2 Appendix 1

Laboratory Procedure for Yeast and Fungi Test Agar

PC/1998/2/3.3 Appendix 1

Laboratory Procedure for Bacterial Test Agar

 

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Part 3: Guidelines for maintaining deposits - Procedures -

Reference nb.

Procedure Title

PC/1998/3/1

Quality Control, Maintenance, Preservation, Storage and Acceptance of Cell Lines - Living Cultures -

PC/1998/3/1.1

Evaluation of Growth Colour and Morphological Characters of Callus Cultures

PC/1998/3/1.2

Standard Extraction Procedure for Callus and Suspension Cultures

PC/1998/3/1.3

Standard HPLC Procedure for Culture Extracts

PC/1998/3/1.4

Measurement of UV Spectra of unpurified Cell Extracts

PC/1998/3/1.5

Preparation HPLC Solvents for Standard Analysis

PC/1998/3/1.6

Description of Methods for Standard HPLC Analysis

 PC/1998/3/2

Quality Control, Maintenance, Preservation, Storage and Acceptance of Cell Lines - Frozen Cultures -

 PC/1998/3/3

Routine Maintenance and Storage of Cell Lines - Living Cell Lines -

PC/1998/3/3.1

Criteria for the Culture Transfer to Fresh Medium out of the Routine Time Schedule

PC/1998/3/3.2

Routine Procedure for the Transfer of Callus Cultures

PC/1998/3/3.3

Routine Procedure for the Transfer of Suspension Cultures to Fresh Medium

 PC/1998/3/4

Routine Maintenance and Storage of Cell Lines - Frozen Cell Lines

PC/1998/3/4.1

Description and Use of the Computer Data Base for Cryocontainer Management

PC/1998/3/4.2

Description and Use of the Cryocontainer Document File (Book)

 

Part 3: Guidelines for maintaining deposits - Appendixes -

Reference Nb.

Appendixes

PC/1998/3/1 Appendix 1

Standard Letter: Cell Line Authentication under Suboptimum Growth Conditions 

PC/1998/3/1 Appendix 2

Standard Letter: Rejection of Cell Line

PC/1998/3/1.2 Appendix 1

Measurement of Dry Weight Percentage of Fresh Cells

PC/1998/3/2 Appendix 1

Standard Letter: Unsuccessful Cryopreservation Procedure

PC/1998/3/2 Appendix 2

Standard Letter: Authentication Check for Thawed and Regrown Cultures

 

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Part 4: Guidelines for delivering material to customers - Procedures -

Reference Nb.

Procedure Title

PC/1998/4/1

Delivery of cell lines

 

Part 4: Guidelines for delivering material to customers - Appendixes -

Reference Nb.

Appendixes

PC/1998/4/1 Appendix 1

Standard Letter: Delivery not possible because of Restrictions

PC/1998/4/1 Appendix 2

Standard Letter: Request of Import Permit

PC/1998/4/1 Appendix 3

Standard Letter: Announcement of Cell Culture Delivery 

 

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Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998; updated August 1999
Page Layout by CERDIC
Copyright CABRI, 1998

© The CABRI Consortium 1999-2013.
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
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