PLANT CELL LINES
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LABORATORY PROCEDURES FOR PLANT CELL LINES
PC/1998/1/7 Appendix 2
«First Aid» after Microbial
(Taken and translated from: Burkhardt:
After any microbial infection in the
laboratory a medical doctor has to be contacted immediately. The following
statements should be taken only as guidelines and should not replace professional
All equipment necessary for «First
Aid» has to be stored in each laboratory a separate container which
has to be clearly marked and which has to be deposited in a dry, cool and
clean place which is all the time accessible for all workers of the laboratory.
Mouth: Never Swallow ! Spit ! Wash
1. Infectious material has
got into the mouth but has not yet been swallowed
Spit immediately. After spitting wash
the mouth and gargle first with freshly made 0.1% KMnO4 solution and afterwards
with 1% H2O2 solution. Avoid swallowing. Spit afterwards. Afterwards stimulate
the acid production of the stomach by eating meat extract. Repeat washing
of the mouth and gargling several times during some hours after the infection.
2. Infectious material has been swallowed
Short but strong washing of the mouth
and gargling with freshly made 0.01% KMnO4 solution. Afterwards stimulate
the acid production of the stomach by eating meat extract and taking pharmaceutical
preparations containing pepsin and/or hydrochloric acid.
Eye: Do not Rub !
3. Infectious material has
got into the eye
Wash the eye and the conjunctival
sap with a solution of 0.0001 % of oxycanate (open the lid and drop the
solution into the eye repeatedly). Apply a salve containing an antibiotic
which is usually used to attack the contaminating microbe.
Microbes may move through the tear
passages into the mouth. Therefore afterwards follow the guidelines described
under topic 1 (mouth) and perform all steps described there.
Nose: Blow you Nose strongly !
Breathe through the Mouth !
4. Infectious material has
got into the nose
Blow your nose repeatedly
strongly into cellulose paper or something similar. Avoid inhaling through
the nose (inhale through the mouth, close the mouth and blow the nose strongly).
Apply an oxycyanate containing salve or solution into the nose.
Perform afterwards the steps described
under topic 1.
Skin: Let open Wounds bleed for
a while ! Disinfect any Wound !
5. Any injury or damage of
the skin by infected instruments, by bites or scrapes of infected or healthy
animals, contamination of skin wounds with infectious material.
Disinfect surface scratches of the
skin immediately with tincture of iodine. Afterwards clean the surroundings
of the wound, swap with cotton and disinfect the wound again with tincture
of iodine and cover it with a protective bandage.
Let a wound from a prick or stab bleed
for a while. Disinfect the puncture with tincture of iodine. Afterwards
cover the puncture with a protective bandage.
Let a strongly bleeding cut bleed
for a while. Disinfect then the surroundings of the wound (not the wound
itself) with tincture of iodine. Protect the wound then with a protective
By injuries with instruments contaminated
with the blood of patients (danger of Hepatitis B or AIDS), or by contaminations
of a wound with anthrax, rabies, Pseudomonas mallei, Tetanus (Clostridium
tetani) or Clostridium perfringens or other extremely dangerous microbes
the contaminating agents have to be killed immediately in the wound. Therefore
in these cases the wound itself should be treated directly with tincture
of iodine as far as possible. Afterwards a hospital has to be contacted
directly and immediately.
After any injury, the injured
limb has to be immobilized for 24 hours.
Guidelines prepared for CABRI by
DSMZ, 20 Jan. 1998
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Copyright CABRI, 1998
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