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LABORATORY PROCEDURES FOR PLANT CELL LINES

Appendix

PC/1998/2/3.3 Appendix 1


Laboratory procedure for bacterial test agar

Tests for bacterial contamination are performed on CASO-Agar. The test agar is prepared as follows:

PROCEDURE

1. The following components are mixed in a 1 l Schott laboratory flask

CASO-Agar (Merck) 20.0 g

Yeast extract 1.5 g

Glucose 1.25 g

Distilled water 500.0 ml

2. Adjust to pH 7.0

3. Autoclave for 30 minutes at 1.2 bar and 120 C

4. Pour into petri dishes (60 - 90 mm in diameter with ventilation) under sterile conditions and allow to solidify.

5. Wrap into a plastic bag and store at 4C.


Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
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Copyright CABRI, 1998 

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