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LABORATORY PROCEDURES FOR MICROORGANISMS

Appendix

M/1998/3.00 Appendix 5.00


Survey of examples of detailed protocols for the different types of preservation methods

CRYOPRESERVATION

BACTERIA

M/1998/3.00 Appendix 5.01
Preservation of bacteria by freezing and low temperature storage in glass capillary tubes

M/1998/3.00 Appendix 5.01.1
Protocol form: Preservation of bacteria in liquid nitrogen

M/1998/3.00 Appendix 5.01.2
Protocol form: Quantity of frozen stock cultures

M/1998/3.00 Appendix 5.01.3
Protocol form: Position of cultures in liquid nitrogen

M/1998/3.00 Appendix 5.02
Preservation of bacteria by freezing and low temperature storage in polypropylene straws

M/1998/3.00 Appendix 5.03
Preservation of sporoactinomycetes by freezing and low temperature storage in PVC straws

M/1998/3.00 Appendix 5.04
Preservation of bacteria above liquid nitrogen in cryotubes

M/1998/3.00 Appendix 5.05
Preservation of bacteria by freezing and low temperature storage below -70C on glass beads

M/1998/3.00 Appendix 5.05.1
Protocol form: Preservation of bacteria by freezing and low temperature storage below -70C on glass beads

M/1998/3.00 Appendix 5.06
Preservation of bacteria on ceramic beads below -70C

 

 

FUNGI

M/1998/3.00 Appendix 5.07
Cryopreservation of fungi

M/1998/3.00 Appendix 5.07.1
Flow Chart: Cryopreservation of fungi

M/1998/3.00 Appendix 5.07.2
Protocol: Cryopreservation of fungi

 

 

 

 

 

 

 

 

 

 

 

 

(FREEZE-)DRYING

BACTERIA

M/1998/3.00 Appendix 5.08
Preservation of bacteria by freeze-drying (1)

M/1998/3.00 Appendix 5.08.1
Flow chart: Freeze-drying process

M/1998/3.00 Appendix 5.08.2
Protocol form: (Freeze)-drying of bacteria

M/1998/3.00 Appendix 5.09
Preservation of bacteria by freeze-drying (2)

M/1998/3.00 Appendix 5.10
Preservation of bacteria by centrifugal freeze-drying

M/1998/3.00 Appendix 5.10.1
Flow chart: Centrifugal freeze-drying

M/1998/3.00 Appendix 5.11
Preservation of bacteria by vacuum-drying

M/1998/3.00 Appendix 5.11.1
Flow chart: Vacuum-drying

M/1998/3.00 Appendix 5.11.2
Protocol form: Predried skim milk batches

M/1998/3.00 Appendix 5.12
Preservation of bacteria by liquid drying (L-drying)

M/1998/3.00 Appendix 5.12.1
Flow chart: L-drying

 

FUNGI

M/1998/3.00 Appendix 5.13
Preservation of fungi by freeze-drying

M/1998/3.00 Appendix 5.13.1
Flow chart: Freeze-drying of fungi

M/1998/3.00 Appendix 5.13.2
Comments: Freeze-drying of fungi


Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998
Page layout by CERDIC
Copyright CABRI, 1998

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