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LABORATORY PROCEDURES FOR MICROORGANISMS

Flow diagram

M/1998/3.00 Appendix 5.07.1


CRYOPRESERVATION OF FUNGI

  

Select procedure

Submerge inocula in cryoprotectant in cryovial

Label cryovial

Cool with selected cooling rate (general 1C/min.) to 45 C

store below -130 C

Check viability and quality after 16 h and 5 year (specialist)

(Revival 5 min. waterbath 30C; Oomycota and sensitive organisms at 25 C)


Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998
Page layout by CERDIC
Copyright CABRI, 1998

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Site maintained by Paolo Romano. Last revised on April 2013.