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Minimum data sets

The Minimum Data Set (MDS) is the set of data that is asked to describe each product from each collection. Each catalogue field has to be filled in (with unknown or not applicable when no information is given).

The Recommended Data Set : collection centres are also asked to supply data for recommended fields; however, if data are not available, the product is still acceptable

Collection centres are free to provide data for other fields, which are part of the Full Data Set

A general MDS can be applied to each type of collections :

The ECACC holds over 1500 cell lines representing 57 different species from exotic sources such as Iguana and red panda to the more common human and rodent. The diversity of the collection is further emphasised by the fact that the 600 human cell lines originate from 48 different tissues.

All cell lines from the collection are tested for absence of microbial contamination (mycoplasma, bacteria, fungi, yeasts) and authenticated by isoenzyme analysis and DNA fingerprint. As a result of funding from the EC BIOMED programme new and existing cancer cell lines in the collection (approximately 250) have been subjected to extensive literature searches resulting in detailed reports on 120 cell lines.

The catalogue is available CD-ROM (MAC and PC), hardcopy and via the internet (http://www.camr.org.uk).

For each cell line in the catalogue there is a full description and detailed culture conditions. For 120 of the cancer cell lines a report is also included with an extended bibliography and sections on cell characteristics and applications (electronic versions only). The electronic versions are fully searchable enabling all fields within each record can be searched e.g. "human chondrosarcoma" or "IL1 and TNF secreting monocytic cell lines"

Order and agreement forms can be edited and printed from the catalogue. Prices list is included.

ECACC number: 85081901
Name: 33B
Description: Rat nervous tissue oligodendroglioma
Culture medium: DMEM+2mM glutamine+10% FBS
Passage number: 55
Subculture routine: Split confluent cultures 1:3 to 1:6 ie seeding
at 2-4x10000 cells/cm2 using trypsin/EDTA;
5% CO2;37°C
Morphology: Neuronal
RCD No
Depositor Dr K Fields,Albert Einstein College ofMedicine,Bronx,New York USA
Reference Neuroscience 1985;15:877-885
Applications Neurobiological studies
Comments Ethyl nitrosourea induced tumour of rat nervous system.Derived from 17th in vivo passage of TR33B;a Ran-1 surface antigen positive line. The monoclonal antibody 217c can be used as marker for normal Schwann & rat glial tumour cells, binds to Ran-1

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BIOMED1 REPORT

The information below has been provided as a result of funding from the EC BIOMED programme. The information has been obtained from literature citing use of this particular cell line. The characteristics have NOT been verified experimentally by ECACC and, therefore, ECACC cannot be held responsible for failure of a cell line to comply to the characteristics listed below.

Cell characteristics:

1) expresses class II NGF receptor (low affinity),i.e. p75NGFR (1,2)
2) has no growth inhibitory activity (3)
3) exhibits phagocytic properties analogous tothose of normal Schwann cells (7,8,9,10)
4) Mycobacterium sp.phagocytosis.Not specific for Mycobacterium leprae (11)
5) binds to MAb 217c via Ran-1 antigen (12)

Applications:
1) Study of class II NGF receptor: binding properties in abscence of class I NGF receptor (2)
2) Screening drugs acting against Mycobacterium leprae: M.leprae is maintained within 33B cell line.(4)
3) Effect of anti-mycobacterial serum on uptake of Mycobacterium by 33B: comparison macrophage/33B cells interaction with Mycobacterium (7)
4) Mycobacterium phagocytosis: comparison with phagocytosis by macrophages (7,8,9,10)
5) Antibody production: can be used as an antigen (13)
6) Studies of regulation of myelin basic protein gene transcription in glial cells (5)

Bibliography:
(1) J Biol Chem 1992;267(32):22707-22710
(2) J Biol Chem 1992;267(20):13917-23
(3) Development 1991;112(1):33-42
(4) Antimicrob Agents Chemother 1991;35(7):1444-7
(5) Biochem Soc Trans 1991;19(2):85S
(6) Exp Cell Res 1989;182(1):173-85
(7) J Neuroimmunol 1987;14(2):235-9
(8) J Neuroimmunol 1986;13(1):109-13
(9) J Neurol Sci 1986;75(1):113-9
(10) Int J Leprosy Other Mycobact Dis 1986;54(2):294-9
(11) Int J Leprosy Other Mycobact Dis 1986;54(1):71-8
(12) Neuroscience 1985;15(3):877-85
(13) Prog Clin Biol Res 1977;15:179-90

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