ACCESSION FORM - PLASMIDS

LABORATORY PROCEDURES FOR PLASMIDS

Appendix

PP/1998/02/01 Appendix 2

ACCESSION FORM - PLASMIDS

NCCB
University of Utrecht
P.O.Box 80.056
3508 TB Utrecht, the Netherlands
tel: +31 (0)30 253 3584
fax: +31 (0)30 251 3655
email: nccb@bio.uu.nl

Please complete the following data:

Name(s) of the plasmid/phage:..................................................................................................

Literature reference:................................................................................................................

Source:

l_lconstructed by own group

l_lreceived on .....................from:.........................................................................................

.................................................................................................................................................

Host organism (species and scientific name, e.g. E. coli DHa ):

.................................................................................................................................................

Culture conditions (e.g.LB+Ap(100), 37°C):................................................................................

.................................................................................................................................................

Remarks:...................................................................................................................................

.................................................................................................................................................

Wish for availability to third parties:

l_lwithout restrictions

l_lfor non-commercial purposes only

Completion (where possible/applicable) of the following data is much appreciated but not required

Information on host:

Literature reference of host:.................................................................................................

............................................................................................................................................

Minimal requirements from host:.........................................................................................

Construction:

Name of parental element:....................................................................................................

Mutation method:.................................................................................................................

Insert(s):..............................................................................................................................

Size of insert:.......................................................................................................................

Remarks:..............................................................................................................................

Reference number in sequence database (GENBANK, EMBL) of:

deposited plasmid:...............................................................................................................

or:

parental plasmids(s):.............................................................................................................

cloned genes/inserts:............................................................................................................

PROPERTIES OF PLASMIDS AND PHAGES

Properties of plasmids:

Molecular weight (in kb):.....................................................................................................

Replicon:.............................................................................................................................

Incompatibility group:..........................................................................................................

Copy number:

l_lmonocopy l_llow (2-5) l_lmedium (5-10)

l_lhigh l_lrunaway l_lremarks:...................................................

Transfer-ability¹:

l_lnon-transferable l_lconjugative l_lmobilizable

l_lremarks:.......................................................................................................................

Antibiotic resistance markers:...............................................................................................

Other markers:.....................................................................................................................

Promoter useful for gene-expression:....................................................................................

Translation initiation region:.................................................................................................

Transcription terminator:......................................................................................................

Remarks:..............................................................................................................................

Properties of phages:

Phage particle size:...............................................................................................................

Pathway of replication¹:

l_llysogenic l_llytic l_lfilamentous phage

l_lremarks:.......................................................................................................................

Cell surface receptor:............................................................................................................

Minimum packing capacity (in kb):.......................................................................................

Maximum packing capacity (in kb):......................................................................................

Nucleic acid type¹:

l_lDNA l_lRNA l_ldouble stranded

l_lsingle stranded l_lcircular l_llinear

Remarks:..............................................................................................................................

VECTOR USES AND APPLICATIONS¹:

l_lgeneral cloning l_lrecombinant vector l_lsuicide vector l_lpositive selection vector l_lexpression vector l_lYAC
l_lreplacement vector l_linsertion vector l_lpromoter probe l_ltransl. initiat. probe l_ltranscription term.probe l_lexport signal probe
l_lsequencing l_lepitope mapping l_l(rec.) protein purification
l_lvector construction (e.g. containing gene casettes with flanking restr. enzyme sites)
l_lother:...........................................................................................................................

Metabolic properties¹:

l_lproduction of:.............................................................................................................

l_ldegradation of:............................................................................................................

l_lresistance to:...............................................................................................................

l_ldegradation of:............................................................................................................

l_lother metabolic properties:..........................................................................................

A. Suicide vector properties:

l_lrandom transposon delivery (random mutagenesis)

l_lconditional replication

l_lmultiple cloning site within IS element

l_lremarks.......................................................................................................................

......................................................................................................................................

B. Expression vector properties¹:

l_lgeneral expression

l_lsecretion vector

l_lheterologous promoter

l_lheterologous. transation initiation region

l_lheterologous messenger stability determinant

l_lin vitro expression: fusion protein

l_lin vitro expression: proteolytic site

l_lother:..........................................................................................................................

......................................................................................................................................

Virus used for¹:

l_lgeneral transduction l_lspecialized transduction l_lplasmid specificity

l_lphage typing l_lother:..............................................................................

Remarks:

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