Guidelines
CELL LINES
PLANT CELL LINES
PLANT VIRUSES
PLASMIDS
PHAGES
MICROORGANISMS
GENOMIC LIBRARIES
PROCEDURES
CATALOGUE
LABORATORY PROCEDURES FOR PLANT CELL VIRUSES
Appendix
PVC/1998/2.03 Appendix 4
CLEAN UP OF MIXED VIRUS INFECTIONS BY SELECTIVE VECTOR TRANSMISSION
INTRODUCTION
Viruses that are transmitted by vectors are specialised with respect to their respective vector species. Different vectors and the different acquisition/retention characteristics of a vector species to transmit viruses to their respective host plants is used to separate mixed virus infections and to obtain pure cultures. Often differential insect transmission is combined with a differential host plant approach
PVC/1998/2.03 Appendix 3.
PROCEDURE
Insect transmissions are essentially done as described in
PVC/1998/2.02 Appendix 4. Viruses belonging to different groups are most conveniently separated by using different vector species. Viruses sharing their insect vector, e.g. potyviruses or luteoviruses, are differentially transmitted by A. persicae allowing various access periods for virus acquisition, e.g. 30 sec. for potyvirus acquisition, to support differential acquisition of the viruses present in a mixed infection. Vector transmission is done by using a group of aphids and by timing virus acquisition of each aphid individually.Guidelines prepared for CABRI by DSMZ, 3 Feb. 1998
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