TORAGE OF THE ORIGINAL VIRUS INFECTED PLANT MATERIALS

LABORATORY PROCEDURES FOR PLANT CELL VIRUSES

REFERENCE NO.: PVC/1998/2.01

TITLE: STORAGE OF THE ORIGINAL VIRUS INFECTED PLANT MATERIALS

INTRODUCTION

Original virus infected plant materials sent to the collection are preserved until the process of authentication is finalised and virus infections are established on appropriate host plants.

PROCEDURE

Upon receipt, plant material infected with a known, tentatively characterised or undescribed virus will be divided in two samples, one for subsequent virus work and a second sample providing backup and being conserved as original plant material.

For conservation of original plant materials, a tentative data sheet is opened, stating sender, date of receipt, plant name and type of material (fresh/dried, leaves/stem cuttings/rootstocks) and putative virus present.

Original material will be kept in liquid nitrogen (PVC/1998/3.01) for the duration of the authentication process (PVC/1998/2.03; PVC/1998/2.04). A final decision is then to be made to keep original material in conservation if:

- material is virus positive but can not be transmitted to alternative host plants

- occurrence of other viruses in original sample that were probably not transmitted is indicated.

Original sample will be destroyed if authentication process was positive.

After finalisation of the authentication, the tentative data entry is converted into an ordinary database entry and all necessary data describing the particular virus are entered.

Guidelines prepared for CARBI by DSMZ, 3 Feb. 1998
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