LABORATORY PROCEDURES FOR MICROORGANISMS
REFERENCE NO.: M/1998/2.05
TITLE: (PRELIMINARY) QUALITY CHECK
From strains sent to the collection for deposit the viability and purity is tested by subculturing the original culture received.
Quality checks for archaea and bacteria:
The following tests are considered as a minimum for preliminary quality check of new deposits:
Ÿ microscopical: cell form cell shape, cell motility, (cell form and cell shape may be documented by photomicrographs)
The preliminary quality check may be extended at this stage or after the preservation of the strain to a "full" quality check which includes more detailed studies of microscopical and macroscopical characteristics, selected physiological, biochemical, chemosystematic properties and DNA studies.
The preliminary quality check may be combined with the application of a commercial characterisation or identification kit (e.g. api, Biolog, or others) to obtain a metabolic profile of the strain studied.
Quality checks for fungi and yeasts:
Minimal quality check
Ÿ microscopical (for bacteria)
Ÿ cultural (e.g. on malt-peptone agar for bacteria)
Sporulation (sterile, moderate, good)
For the characterisation of fungi and yeasts some of the following tests must be performed:
Ÿ Microscopical: Sexual spores, asexual spores, hyphal characteristics
Ÿ pH tolerance
Ÿ specific enzyme tests
Ÿ production of specific metabolites
Ÿ cell wall composition
Ÿ metabolic patterns
Ÿ protein patterns
Ÿ fatty acids
In case the material fails the tests, the material is
In case the material passes the test an accession number is assigned to it (if this was not already done earlier) and the material is preserved (M/1998/3.00)
Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998; updated August 1999
© The CABRI Consortium 1999-2013.