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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

  

Quality controls at INRC

 

Identification of cell line

Mycoplasma testing

Reference Number

Procedure Title

Reference Number

Procedure Title

AHC/1998/3/3.1/2.1

Cell enumeration by vital dye exclusion

AHC/1998/3/3.2/2.1

Mycoplasma detection by Hoescht stain

AHC/1998/3/3.1/2.2

Isoenzyme analysis

AHC/1998/3/3.2/3.1

Mycoplasma detection by nested double step PCR

AHC/1998/3/3.1/3.1

Species confirmation by direct analysis of restriction digests of genomic DNA

AHC/1998/3/3.2/3.2

Mycoplasma detection by the GibcoBRL Mycotect

AHC/1998/3/3.1/3.2

Species confirmation for human cell lines by HLA DQ Alpha amplification

AHC/1998/3/3.2/1.5

Mycoplasma eradication

 

Bacteria and fungi testing

Reference Number

Procedure Title

AHC/1998/3/3.3/2.1

Bacteria and Fungi detection by culture

AHC/1998/3/3.3/2.2

Bacteria and Fungi eradication

 


Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
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