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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Quality controls at ECACC

 

Identification of cell line

Mycoplasma testing

Reference Number

Procedure Title

Reference Number

Procedure Title

AHC/1998/3/3.1/2.1

Cell enumeration by vital dye exclusion

AHC/1998/3/3.2/2.1

Mycoplasma detection by Hoescht stain

AHC/1998/3/3.1/2.2

Isoenzyme analysis

AHC/1998/3/3.2/2.2

Mycoplasma detection by culture

AHC/1998/3/3.1/2.3

DNA fingerprinting

AHC/1998/3/3.2/1.5

Mycoplasma eradication

 

Bacteria and fungi testing

Virus testing

Reference Number

Procedure Title

Reference Number

Procedure Title

AHC/1998/3/3.3/2.1

Bacteria and Fungi detection by culture

AHC/1998/3/3.4/2.1

Isolation of cell line RNA for use in RT-PCR

AHC/1998/3/3.3/2.2

Bacteria and Fungi eradication

AHC/1998/3/3.4/2.2

BVDV detection by use of RT-PCR

AHC/1998/3/3.4/2.3

BVDV detection by use of a DiG labelled probe following RT PCR

AHC/1998/3/3.4/2.4

PCR analysis for HIV

AHC/1998/3/3.4/2.5

PCR analysis for HTLV 1,2

 

 


Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
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