Home

Description

Search Catalogues

Browse catalogues

Collections

Prices

Guidelines

Search Web Site

Contacts

FAQ

Site Map

LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

REFERENCE NO: AHC/1998/3/3.4/1.5


TITLE: RETROVIRUS DETECTION BY ELISA


In the ELISA, digoxigenin- and biotin-labeled desoxyuridine-triphosphates are incorporated in the presence of viral reverse transcriptase (RT) during the synthesis of a DNA strand along a synthetic target of single-stranded DNA molecules. The newly synthesized DNA is trapped on streptavidin-coated microtiter plates and subsequently detected immunologically by binding of peroxidase-labeled anti-digoxigenin antibodies and visualized by color development and finally quantified by measuring the bsorbance with an ELISA reader (1). Two different buffers are used to detect Mg2+-dependent RT as well as Mn2+-dependent RT of C-type retroviruses.

References:

1. Eberle J, Seibl R: A new method for measuring reverse transcriptase activity by ELISA. J Virol Meth 40: 347-356 (1992).


Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
Page layout by CERDIC
Copyright CABRI, 1998

© The CABRI Consortium 1999-2013.
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on April 2013.