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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Appendix

REFERENCE NO: AHC/1998/3/1.2 Appendix 4


TITLE: INACTIVATION OF FOETAL BOVINE SERUM


INTRODUCTION

Inactivated foetal bovine serum, FBS, is used for making up media.

PROCEDURE

  1. Take bottle of FBS and warm to 37 1C.
  2. Heat water bath to 56 1C.
  3. Place warmed bottle of FBS in water bath at 56 1C for 30 minutes.
  4. Remove and allow bottle to cool.

 

Spray bottle with 10% alcohol before taking to tissue culture cabinet.


Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
Page layout by CERDIC
Copyright CABRI, 1998

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