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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Appendix

REFERENCE NO: AHC/1998/3/1.2 Appendix 2


TITLE: CELL CULTURE MEDIA PREPARATION FORM - ECACC -


Name:

 

Date:

 

Extension No:

 

Date Required:

(min of 1 week after date requested)

 

Media Required:

 

Frequency:

 

Quantity:

 

 

 

Additional Reagents

FBS

Inactivated:

YES/NO

 

 

 

Concentration:

 

No of Bottles:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Supplements (tick if required)

NEAA o Pyruvate o

Vitamins o Mercaptoethanol o

Hepes o HT o

HAT o Pen/Strep o

Aminopterin o Kanamycin o

Gentamycin o Neomycin o

Nystatin o

Others (give details below)

Supplement

Concentration

 

 

 

  

  

 

 

 

NB: Media is supplied in 500ml bottles - and is normally made up in batches of 10. Please try and request your media in multiples of these quantities.

 

(To be completed by the operator preparing the medium)

Collection Batch No:

 

 

 

Type of Medium:

 

Manufacturer:

 

Batch No:

 

Expiry Date:

 

No. of bottles rejected:

 

and reason:

 

No. of bottles prepared for use:

 

 

 

Type(s) serum:

Manufacturer(s):

Batch No(s):

(FBS)

Expiry date(s):

 

No of bottles used:

(FBS)

 

 

Volume of serum/500ml medium:

(FBS)

 

 

L-Glutamine batch No:

 

Manufacturer:

 

Expiry date:

 

 

 

Volume of Glutamine/500ml medium:

 

Non-essential amino acids batch no:

 

Manufacturer:

 

Expiry date:

 

Volume of NEAA/500ml medium:

 

Other additions made:

 

Details (i.e. batch No., Manufacturer etc.):

 

 

  

QC OF PREPARED MEDIUM

Batch number of TSB: ......................................................................... No of TSB Broths used ......................... incubated at 37 1C ..........R.T.........

Batch number of Thioglycollate broths: ....................................................

No of Thioglycollate broths used ............. incubated at 37 1C ...........R.T.........

Operator .......................................... Date ...........................

 

QC Results after ............ days incubation

All QC'd OK ...... (tick) or:

Individual bottle QC checks (if taken before end of normal QC times)

Bottle No:

Date:

QC (initial) 

 

 

 

 

 

 

 

 

QC No(s) suspect/contaminated................................................................................................................

Indicate incubation temperatures in brackets after bottle No(s) or

Whole batch contaminated ........... (tick) and reject

Medium passed for laboratory use by:.......................................... Date ........................


Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
Page layout by CERDIC
Copyright CABRI, 1998

© The CABRI Consortium 1999-2013.
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on April 2013.